[Evaluation of CT scan guided fine needle biopsy of the chest lesions]

Background: CT scan guided fine needle biopsy of the chest lesions are increasingly popular technique, but may have complications which the most common is pneumothorax. The purpose of this study was to observe the complications of needle biopsy of chest lesions guided by the CT scan among the patients studied

Material and Methods: This is a descriptive epidemiologic study. All patients that referred to Imam Khomeini in Ahvaz from 2005 to 2007and it was necessary to biopsy the chest lesions were studied. Snapshot files on lung CT scan lesion size and depth measurements and the biopsy samples obtained were sent to pathology. After the biopsy the patient CT scan was to act as the presence or absence of symptoms, proper action was made of

Results: Among 102 patients studied, 85 patients [83.4%] were uncomplicated, but 8 patients [7.9%] were suffered from pneumothorax. In the study were made between lesion size and depth of complications found no correlation, but between lesion location and complications caused there was statistically significant difference

Conclusion: the needle biopsy guided by CT scan due to low complications in many cases is a suitable diagnostic method

[ effect of oxamate on LDH-C4 activity of sperm in rats]

LDH-C4 is one of the lactate dehydrogenase isoenzymes found in mature testis and spermatozoa of many species. The Physiological function of these isoenzymes indicates its role in creating energy for sperm motility and survival. In this research, the effects of oxamate as specific inhibitors of the LDH-C4 of rat were studied in vivo. A total of 20 adult rats were divided into four equal groups. One group as the control group received saline only, and different amounts of oxamate were injected into other three groups [600, 300, 150mg/kg] daily for 45 days intraperitoneally. The rats were then killed with chloroform and the caudal part of epididym was separated. By making several cuts in caudal part of the epididymis, the sperms were isolated and put in T6 medium+5mg/ml[-1] BSA. Later, the sperms were incubated under 37§C and 5% CO2 for one hour. LDH-C4 enzyme was extracted using the Erwin Goldberg and the protein amounts were measured by Lowry's method. Relative purification was done in two stages including ammonium sulfate precipitation and column chromatography by DEAE-Sephadex-A50. All stages of extraction, the amount of total protein, LDH enzyme activity in the oxamate-exposed groups, and specified recipient, were then compared with the control group. In this study, the total enzyme LDH-C4 activity in the control group was 11.8 +/- 0.3 and the oxamate recipient groups [150, 300 and 600mg/kg] were 8.3 +/- 0.3, 6.9 +/- 0.2 and 3.2 +/- 0.1 IU, respectively. The concentration of 600mg/kg oxamate was inhibited about 63% enzyme activity compared to control group. This study showed that the oxamate may reduce in vivo enzyme activity through LDH-C4 with increasing concentration and this effect is proportional. Therefore, with the effect of the competitive inhibitors of oxamate on LDH-C4, this substrate can be used as a contraceptive for males

[ effect of unilateral testicular blunt trauma and protective effect of zinc on spermatogenesis of contra lateral testis of pre-pubertal wistar rate]

There has so far been controversy among researches about the effect of unilateral blunt testis trauma on structure and function of contra lateral testis. This study was designed to evaluate the effect of unilateral blunt testicular trauma after a time span of spermatogenesis [40 days] on the number of round and elongated spermatic cells, spermatogonia, primary spermatocyte, sertoli and leydig cells of contra-lateral testis as well as to assess the protective effect of zinc on unilateral blunt testicular trauma. In this experimental study 30 pre-pubertal male Wistar rats [age 7 weeks] were divided into three equal groups [n=10]. Group I [control] underwent a sham operation of the left testis under general anesthesia. Rats in group II and group III [with blunt testicular trauma] were subjected to left blunt testicular trauma to rupture the tunica albuginea. In group III just after the trauma 30 mg/kg zinc sulfate were given intra-peritoneally followed by admission of Zn for 40 days at a dose of 500 parts per million [ppm] /day orally. Later on, all rats were sacrificed and their right testes were removed and after histological processing and staining by H and E method the samples were evaluated by counting round and elongated spermatid cells, spermatogonia, primary spermatocyte, sertoli and leydig cells and also by cell structures study. Data were analyzed using ANOVA, Tukey and Duncan's tests and pvalue of <0.05 was considered as statistically significant. There was no significant differences in the number of round and elongated spermatid, spermatogonia, sertoli and leydig cells of testis if the three groups [P>0.05]. Histological changes in sertoli and leydig cell were seen. Therefore, the protective effect of zinc was not significant on germ cell proliferation and structure. The results suggest that unilateral blunt testis trauma after time course of 40 days has no effect on germ cell layers and leydig and sertoli cells proliferation. This study suggests long term evaluation of testes after blunt trauma with use of electron microscope